CD79b Protein (AA 29-159) (His-Avi Tag,Biotin)

Produktnummer ABIN7274235

Dieses Recombinant CD79b-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für CD79b Protein (AA 29-159) (His-Avi Tag,Biotin) (ABIN7274235)

Target: CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 29-159

Aufreinigungstag / Konjugat: Dieses CD79b Protein ist gelabelt mit His-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Human CD79B Protein

Sequenz: Ala29-Asp159

Produktmerkmale: Recombinant Biotinylated Human CD79B Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala29-Asp159.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CD79B Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD79B, His Tag with the EC50 of 23.3ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))

Andere Bezeichnung: CD79B

Hintergrund: CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta-chain) is a 36-40 kDa member of the Ig-Superfamily. It is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.

Molekulargewicht: 18.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

UniProt: P40259-1

Pathways: BCR Signaling