CD80 Protein (CD80) (AA 35-242) (His-Avi Tag)

Produktnummer ABIN7273893

Dieses Recombinant CD80-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für CD80 Protein (CD80) (AA 35-242) (His-Avi Tag) (ABIN7273893)

Target: CD80

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 35-242

Aufreinigungstag / Konjugat: Dieses CD80 Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Non-biotinylated Human B7-1/CD80 Protein

Sequenz: Val35-Asn242

Produktmerkmale: Recombinant Human B7-1/CD80 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val35-Asn242.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).,Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test).,Human B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of- Human B7-1 is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CD80

Andere Bezeichnung: B7-1

Hintergrund: Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.

Molekulargewicht: 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

UniProt: P33681

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints