ApoE3 Protein (AA 19-317) (His tag)

Produktnummer ABIN7273865

Recombinant ApoE3-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für ApoE3 Protein (AA 19-317) (His tag) (ABIN7273865)

Target: ApoE3 (Apolipoprotein E3 (ApoE3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 85 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 19-317

Aufreinigungstag / Konjugat: Dieses ApoE3 Protein ist gelabelt mit His tag.

Verwendungszweck: Human APOE3/Apolipoprotein E Protein

Sequenz: Lys19-His317

Produktmerkmale: Recombinant Human APOE3/Apolipoprotein E Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Lys19-His317.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human APOE3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human APOE3 is greater than 85% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: ApoE3 (Apolipoprotein E3 (ApoE3))

Andere Bezeichnung: APOE3

Hintergrund: Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).

Molekulargewicht: 35.3 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism