Angiopoietin 2 Protein (ANGPT2) (AA 275-496) (His-Avi Tag)

Produktnummer ABIN7273860

Dieses Recombinant Angiopoietin 2-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für Angiopoietin 2 Protein (ANGPT2) (AA 275-496) (His-Avi Tag) (ABIN7273860)

Target: Angiopoietin 2 (ANGPT2)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 275-496

Aufreinigungstag / Konjugat: Dieses Angiopoietin 2 Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Non-biotinylated Human ANGPT2/Angiopoietin-2 Protein

Sequenz: Lys275-Phe496

Produktmerkmale: Recombinant Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Lys275-Phe496.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human TIE2, His Tag immobilized on CM5 Chip can bind Human ANGPT2, His Tag with an affinity constant of 2.38 uM as determined in SPR assay (Biacore T200).,Immobilized Human ANGPT2, His Tag at 2ug/ml (100ul/well). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA (QC Test).,Human ANGPT2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human ANGPT2 is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Angiopoietin 2 (ANGPT2)

Andere Bezeichnung: ANGPT2

Hintergrund: Angiopoietin-2 (Ang-2, also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.

Molekulargewicht: 28.4 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.

UniProt: O15123

Pathways: RTK Signalweg