Annexin A2 Protein (ANXA2) (AA 2-339) (His tag)

Produktnummer ABIN7273846

Produktdetails

Target: Annexin A2 (ANXA2)

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-339

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses Annexin A2 Protein ist gelabelt mit His tag.

Verwendungszweck: Mouse ANXA2 Protein

Sequenz: Ser2-Asp339

Produktmerkmale: Recombinant Mouse ANXA2 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ser2-Asp339.

Reinheit: > 95 % as determined by Tris-Bis PAGE

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM MES, 150 mM NaCl, 200 mM L-arginine, 5 mM DTT ( pH 5.5).

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM MES, 150 mM NaCl, 200 mM L-arginine, 5 mM DTT ( pH 5.5). Normally 8 % trehalose is added as protectant before lyophilization.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Annexin A2 (ANXA2)

Andere Bezeichnung: ANXA2 (ANXA2 Produkte)

Hintergrund: ANXA2, highly expressed in invasive breast cancer cells, is closely related with poor prognosis, and acts as a molecular switch to EGFR activation. ANXA2 expression is inversely correlated with cell sensitivity to gefitinib. Knockdown of ANXA2 expression in MDA-MB-231 cells increased the gefitinib induced cell death. When ANXA2 was overexpressed in MCF7 cells, the gefitinib induced cell death was decreased. Furthermore, the phosphorylation of ANXA2 at Tyr23 is negatively correlated with the sensitivity of TNBC to gefitinib.

Molekulargewicht: 39.6 kDa same as Tris-Bis PAGE result.

UniProt: P07356

Pathways: S100 Proteine