beta Amyloid Protein

Produktnummer ABIN7272125

Dieses Synthetic beta Amyloid-Protein wird in Synthetic produziert.

Kurzübersicht für beta Amyloid Protein (ABIN7272125)

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Protein-Typ: Synthetic

Spezies: Human

Quelle: Synthetic

Applikation: Western Blotting (WB), In vitro Assay (in vitro), In vivo Studies (in vivo)

Reinheit: >95% pure using mass spec and HPLC.

Produktdetails

Verwendungszweck: Human Synthetic Amyloid Beta Peptide 1-42 (HFIP treated) Monomers

Sequenz: DAEFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVV IA

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -80 °C

Informationen zur Lagerung: -80°C

Antigendetails

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Andere Bezeichnung: Amyloid Beta

Hintergrund: Our amyloid beta peptide 1-42 (Aβ42) is produced synthetically and treated with 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) prior to drying which breaks down pre-formed fibrils and monomerizes the peptide, as previously published (1,2). Upon resuspension in DMSO/dH2O, our Aβ42 presents as a monomeric peptide without fibrils when observed under TEM, AFM and on a Western Blot with an anti-amyloid beta antibody. In contrast to AB42 oligomer and fibril constructs, our Aβ42 monomers were not toxic to primary rat cortical neurons. In the brain, amyloid beta peptide (Aβ) is generated by protease cleavage of amyloid precursor protein (APP), which aggregates into oligomers, protofibrils, fibrils and ultimately plaques in neurodegenerative diseases. The accumulation of Aβ plaques in the brain is considered a hallmark of Alzheimer's disease (AD), and most of the drugs tested for AD in the past 20 years have targeted amyloid beta accumulation (3). Soluble Aβ oligomers isolated from the brains of AD patients or those generated in vitro potently impaired synapse structure and function (4). Aβ oligomers generated in vitro were toxic to PC12 cells (2) and SH-SY5Y cells (5). Aβ was demonstrated to interact with tauopathies to affect neurodegeneration in AD patients (6) and accumulations of Aβ were shown to be associated with lower survival rates in Parkinson's disease patients with dementia (7)., cellular localisation: Cell Membrane , Intracellular Vesicles

Molekulargewicht: 4.5 kDa

Gen-ID: 351

UniProt: P05067

Pathways: Inflammasome