CXCL12 Protein

Produktnummer ABIN7232731

Recombinant CXCL12-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für CXCL12 Protein (ABIN7232731)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Biochemical Assay (BCA), Scintillation Proximity Assay (SPA)

Reinheit: > 97 %

Produktdetails

Verwendungszweck: Recombinant human CXCL12a is produced in E. coli

Sequenz: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQV CIDPKLKWIQ EYLEKALNKM olecular Mass7.98kDa by Mass Spec.Purity>97%ActivityEC 50 = 0.62nM determined by Calcium Flux with human CXCR4 cells.SPA Binding assay of CXCL12/SDF-1a to CXCR7 (IC50=14pM) and CXCR4 (IC50=2nM).Endotoxin Level<0.01 EU per 1ug of protein by LAL method.FormLyophi lized.Carrier ProteinNon e.

Produktmerkmale: Recombinant human CXCL12a is produced in E. coli

Biological Activity Comment: EC50 = 0.62nM determined by Calcium Flux with human CXCR4 cells.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge tube prior to resuspending. Recommended at 100 μg/mL in sterile distilled water.

Lagerung: 4 °C,-20 °C,-80 °C

Antigendetails

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Andere Bezeichnung: CXCL12a

Hintergrund: Stromal cell-derived factor 1,CXCL12a and CXCL12b, also known as Stromal Cell-Derived Factor 1a and 1b (SDF-1a and SDF-1b), are small cytokines that belong to the intercrine family. Both forms of CXCL12 are produced by alternate splicing of the same gene. CXCL12 is strongly chemotactic for T- lymphocytes and monocytes, and it plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism. It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumor progression. CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12. In breast cancer, however, increased expression of CXCL12 is associated with a reduced risk of distant metastasis. By blocking CXCR4, a major coreceptor for HIV-1 entry, CXCL12 acts as an endogenous inhibitor of CXCR4-tropic HIV-1 strains. CXCL12 was shown to be expressed in many tissues in mice including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow.,rHuCXCL12a, SDF-1alpha

Molekulargewicht: 7.98 kDa

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling