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ZEBOV GP Protein

Recombinant ZEBOV GP-Protein exprimiert in HEK-293 Cells.
Produktnummer ABIN7198929

Kurzübersicht für ZEBOV GP Protein (ABIN7198929)

Target

ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))

Protein-Typ

Recombinant

Spezies

  • 3
  • 1
Ebola Virus

Quelle

  • 2
  • 1
  • 1
HEK-293 Cells

Reinheit

> 95 % as determined by SDS-PAGE.
  • Verwendungszweck

    Recombinant EBOV (subtype Zaire, strain Mayinga 1976) GP-RBD / Glycoprotein Protein

    Sequenz

    Met1-Phe308

    Produktmerkmale

    A DNA sequence encoding the Zaire ebolavirus (strain Mayinga 1976) GP (AAC54887.1) (Met1-Phe308) was expressed with five amino acids (DDDDK) at the C-terminus.

    Endotoxin-Niveau

    < 1.0 EU per μg protein as determined by the LAL method.
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  • Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Please refer to the printed manual for detailed information.

    Buffer

    Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.

    Lagerung

    4 °C,-20 °C,-80 °C

    Informationen zur Lagerung

    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target

    ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))

    Andere Bezeichnung

    ZEBOV GP

    Hintergrund

    The fourth gene of the EBOV genome encodes a 16- kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.

    Molekulargewicht

    31.5kDa
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