ACE2 Protein (His tag)

Produktnummer ABIN7321043

Produktdetails

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Rhesusaffen

Quelle: Human Cells

Aufreinigungstag / Konjugat: Dieses ACE2 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Rhesus Macaque Angiotensin-Converting Enzyme 2/ACE-2 (C-10His)

Sequenz: Gln18-Val739

Produktmerkmale: Recombinant Rhesus Macaque Angiotensin-Converting Enzyme 2 is produced by our Mammalian expression system and the target gene encoding Gln18-Val739 is expressed with a 10His tag at the C-terminus.

Reinheit: >95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Rhesus Macaque ACE-2-His(Cat#PKSQ050119)at 5μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-SD1-mFc(Cat#PKSR030476). The ED50 of Recombinant 2019-nCoV S Protein RBD-SD1-mFc(Cat#PKSR030476) is 16.8 ng/ml .

Anwendungsinformationen

Kommentare:

90-120 kDa

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 150 mM NaCl, 1 mM ZnCl2, pH 7.5.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Andere Bezeichnung: ACE-2 (ACE2 Produkte)

Hintergrund:

Background: Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don't inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.

Synonym: Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-Converting Enzyme Homolog, ACEH, Metalloprotease MPROT15, ACE2

Molekulargewicht: 85.1 kDa

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour