Tyrosine Hydroxylase Protein (TH) (His tag)

Produktnummer ABIN7320079

Recombinant Tyrosine Hydroxylase-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für Tyrosine Hydroxylase Protein (TH) (His tag) (ABIN7320079)

Target: Tyrosine Hydroxylase (TH)

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Reinheit: > 85 % as determined by SDS-PAGE

Produktdetails

Aufreinigungstag / Konjugat: Dieses Tyrosine Hydroxylase Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse TH/Tyrosine Hydroxylase Protein (His Tag)

Sequenz: Pro 2-Ser 498

Produktmerkmale: A DNA sequence encoding the mouse TH (P24529) (Pro 2-Ser 498) was fused with a polyhistidine tag at the N-terminus.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Tyrosine Hydroxylase (TH)

Andere Bezeichnung: TH/Tyrosine Hydroxylase

Hintergrund:

Background: Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.

Synonym: TH

Molekulargewicht: 58 kDa

UniProt: P24529

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour