TEK Protein (AA 771-1124) (His-GST)

Produktnummer ABIN7317790

Dieses Recombinant TEK-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für TEK Protein (AA 771-1124) (His-GST) (ABIN7317790)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: ELISA

Reinheit: > 92 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 771-1124

Aufreinigungstag / Konjugat: Dieses TEK Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human Tie2/CD202b Protein (His & GST Tag)

Sequenz: Gln771-Ala1124

Produktmerkmale: A DNA sequence encoding the human TEK (NP_000450) (Gln771-Ala1124) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. No Kinase Activity. 2. Immobilized human TEK (aa 771-1124) at 2 μg/ml (100 μl/well) can bind human Ang2-Fc with a linear range of 0.31-20 μg/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Andere Bezeichnung: Tie2/CD202b

Hintergrund: CD202B,TIE-2,TIE2,VMCM,VMCM1,TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Molekulargewicht:

Calculated MW: 68.3 kDa

Observed MW: 64 kDa

Gen-ID: 7010

NCBI Accession: NP_000450

UniProt: Q02763

Pathways: RTK Signalweg, Growth Factor Binding