ErbB2/Her2 Protein

Produktnummer ABIN7321221

Recombinant ErbB2/Her2-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für ErbB2/Her2 Protein (ABIN7321221)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: HEK-293 Cells

Reinheit: > 92 % as determined by SDS-PAGE

Produktdetails

Verwendungszweck: Recombinant Rat HER2/ErbB2 Protein (Active)

Sequenz: Met 4-Thr 656

Produktmerkmale: A DNA sequence encoding the rat ErbB2 (AAH61863.1) extracellular domain (Met 4-Thr 656) was expressed and purified, with seven additional amino acids (ALEVLFQ) at the C-terminus.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. 2.Immobilized rat ErbB2 at 10μg/mL (100μL/well) can bind herceptin, the EC50 of herceptin is 30-90ng/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Andere Bezeichnung: HER2/ErbB2

Hintergrund:

Background: Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: ErbB2;Neu;p185erbB2

Molekulargewicht: 70.6 kDa

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Skeletal Muscle Fiber Development