Pleiotrophin Protein (PTN)

Produktnummer ABIN7197415

Dieses Recombinant Pleiotrophin-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für Pleiotrophin Protein (PTN) (ABIN7197415)

Target: Pleiotrophin (PTN)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Reinheit: > 95 % as determined by SDS-PAGE

Produktdetails

Verwendungszweck: Recombinant Mouse Pleiotrophin/PTN/HB-GAM Protein (Active)

Sequenz: Met1-Asp168

Produktmerkmale: A DNA sequence encoding the mouse PTN (P63089) (Met1-Asp168) was expressed.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized mouse PTN at 10 μg/ml (100 μl/well) can bind rat SDC1-Fc , The EC50 of rat SDC1-Fc is 0.4-1.1 μg/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 1M NaCl, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Pleiotrophin (PTN)

Andere Bezeichnung: Pleiotrophin/PTN/HB-GAM

Hintergrund:

Background: HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.

Synonym: Pleiotrophin, PTN, Heparin-binding brain mitogen, HBBM, Heparin-binding growth factor 8, HBGF-8, Osteoblast-specific factor 1, OSF-1,HARP,HB-GAM,HBBN,HBNF,OSF,Osf-1,Osf1

Molekulargewicht: 15.3 kDa

UniProt: P63089

Pathways: RTK Signalweg