Nemo-Like Kinase Protein (NLK) (His-GST)

Produktnummer ABIN7317069

Dieses Recombinant Nemo-Like Kinase-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für Nemo-Like Kinase Protein (NLK) (His-GST) (ABIN7317069)

Target: Nemo-Like Kinase (NLK)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 91 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses Nemo-Like Kinase Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human NLK Protein (His & GST Tag)

Sequenz: Val121-Glu 527

Produktmerkmale: A DNA sequence encoding the human NLK (Q9UBE8) (Val121-Glu527) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 3 nmol/min/mg using MBP as substrate.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: Nemo-Like Kinase (NLK)

Andere Bezeichnung: NLK

Hintergrund: LAK1,NLK,Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, nemo-like kinase regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.

Molekulargewicht:

Calculated MW: 74.1 kDa

Observed MW: 73 kDa

Gen-ID: 51701

UniProt: Q9UBE8

Pathways: Ubiquitin Proteasome Pathway