NEK7 Protein (NEK7) (His-GST)

Produktnummer ABIN7317545

Recombinant NEK7-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für NEK7 Protein (NEK7) (His-GST) (ABIN7317545)

Target: NEK7

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses NEK7 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human NEK7 Protein (His & GST Tag)

Sequenz: Met 1-Ser 302

Produktmerkmale: A DNA sequence encoding the human NEK7 (NP_598001.1) (Met1-Ser 302) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.5, 0.5 mM Reduced Glutathione, 0.5 mM PMSF
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Konservierungsmittel: PMSF

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: NEK7

Andere Bezeichnung: NEK7

Hintergrund: NEK7,NIMA (never in mitosis gene a)-related kinase 7, NEK7 belongs to the NIMA subfamily, NEK Ser/Thr protein kinase family, protein kinase superfamily. NEKs (NIMA-related kinases) are mammalian serine/threonine (Ser/Thr) protein kinases structurally related to Aspergillus NIMA (Never in Mitosis, gene A), which plays essential roles in mitotic signaling. NEKs share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent carboxyl-terminal tails of varying length. NEKs are commonly referred to as mitotic kinases, although a definitive in vivo verification of this definition is largely missing. Reduction in the activity of NEK7 or its close paralog, NEK6, has previously been shown to arrest cells in mitosis, mainly at metaphase. NEK7 is a regulator of cell division, and reveal it as an essential component for mammalian growth and survival. The intimate connection between tetraploidy, aneuploidy and cancer development suggests that NEK7 deregulation can induce oncogenesis. The endogenous NEK7 protein is enriched at the centrosome in a microtubule-independent manner. Overexpression of wt or kinase-defective NEK7 resulted in cells of rounder appearance, and higher proportions of multinuclear and apoptotic cells.

Molekulargewicht:

Calculated MW: 62.4 kDa

Observed MW: 58 kDa

NCBI Accession: NP_598001

UniProt: B2R8K8

Pathways: Inflammasome