MAPKAP Kinase 5 Protein (His-GST)

Produktnummer ABIN7317049

Recombinant MAPKAP Kinase 5-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für MAPKAP Kinase 5 Protein (His-GST) (ABIN7317049)

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 76 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses MAPKAP Kinase 5 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human MAPKAPK5 Protein (His & GST Tag)

Sequenz: Met 1-Gln 471

Produktmerkmale: A DNA sequence encoding the human MAPKAPK5 isoform 1 (NP_003659.2) (Met 1-Gln 471) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Andere Bezeichnung: MAPKAPK5

Hintergrund: MAPKAP-K5,MK-5,MK5,PRAK,MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novelin vitrosubstrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation.

Molekulargewicht:

Calculated MW: 82.0 kDa

Observed MW: 75 kDa

Gen-ID: 8550

NCBI Accession: NP_003659

UniProt: Q8IW41

Pathways: MAPK Signalweg