LTA4H Protein (His tag)

Produktnummer ABIN7320340

Dieses Recombinant LTA4H-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für LTA4H Protein (His tag) (ABIN7320340)

Target: LTA4H (Leukotriene A4 Hydrolase (LTA4H))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Reinheit: > 92 % as determined by SDS-PAGE

Produktdetails

Aufreinigungstag / Konjugat: Dieses LTA4H Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse LTA4H Protein (His Tag)(Active)

Sequenz: Met 1-Asp 611

Produktmerkmale: A DNA sequence encoding the mouse LTA4H (NP_032543.2) (Met 1-Asp 611) was expressed, with a C-terminal polyhistidine tag.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC). The specific activity is >15 pmoles/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: LTA4H (Leukotriene A4 Hydrolase (LTA4H))

Andere Bezeichnung: LTA4H

Hintergrund:

Background: Leukotriene A-4 hydrolase, also known as LTA-4 hydrolase, Leukotriene A (4) hydrolase, LTA4H and LTA4, is cytoplasm protein which belongs to the peptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may in turn be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.

Synonym: Lta4h

Molekulargewicht: 70.4 kDa

NCBI Accession: NP_032543