IL-7 Protein (AA 26-177) (His tag)

Produktnummer ABIN7319545

Recombinant IL-7-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für IL-7 Protein (AA 26-177) (His tag) (ABIN7319545)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Cell Culture (CC)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 26-177

Aufreinigungstag / Konjugat: Dieses IL-7 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Interleukin-7/IL-7 Protein

Sequenz: Asp26-His177

Produktmerkmale: Recombinant Human Interleukin-7 is produced by our E.coli expression system and the target gene encoding Asp26-His177 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC). The ED₅₀ for this effect is <0.8 ng/mL. The specific activity of recombinant human IL-7 is > 1 x 10⁸ IU/mg.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Andere Bezeichnung: Interleukin-7/IL-7

Hintergrund: Lymphopoietin,IL7,IL-7,IL 7,Interleukin 7,Interleukin-7,LP-1,Lymphopoietin 1,Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65 % sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT, which regulate lymphocyte survival, glucose uptake, proliferation, and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.

Molekulargewicht:

Calculated MW: 18.3 kDa

Observed MW: 19 kDa

Gen-ID: 3574

UniProt: P13232

Pathways: JAK-STAT Signalweg