GZMB Protein (His tag)

Produktnummer ABIN7195987

Dieses Recombinant GZMB-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für GZMB Protein (His tag) (ABIN7195987)

Target: GZMB (Granzyme B (GZMB))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 97 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses GZMB Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Granzyme B/GZMB Protein (His Tag)

Sequenz: Met 1-Tyr 247

Produktmerkmale: A DNA sequence encoding the proform of human Granzyme B (NP_004122.1) (Met 1-Tyr 247) was expressed with a C-terminal polyhistidine tag.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: GZMB (Granzyme B (GZMB))

Andere Bezeichnung: Granzyme B/GZMB

Hintergrund: CCPI,CGL-1,CGL1,CSP-B,CSPB,CTLA1,CTSGL1,HLP,SECT,Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.

Molekulargewicht:

Calculated MW: 27.0 kDa

Observed MW: 36 kDa

NCBI Accession: NP_004122

Pathways: Apoptose, Caspase Kaskade in der Apoptose