FGF1 Protein

Produktnummer ABIN7318475

Produktdetails

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Verwendungszweck: Recombinant Human FGF-1/FGFa Protein (Active)

Sequenz: Phe16-Asp155

Produktmerkmale: Recombinant Human Fibroblast growth factor 1/Fibroblast Growth Factor Acidic is produced by our E.coli expression system and the target gene encoding Phe16-Asp155 is expressed.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.2-2 ng/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 400 mM NaCl, 1 mM DTT, pH 8.0 .

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Andere Bezeichnung: FGF-1/FGFa (FGF1 Produkte)

Hintergrund:

Background: FGF acidic, also known as ECGF, FGF-1and HBGF-1, is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. It is a mitogenic peptide that is produced by multiple cell types and stimulates the proliferation of cells of mesodermal, ectodermal, and endodermal origin. Its association with heparan sulfate is a prerequisite for activation of FGF receptors. Internalized FGF acidic migrates to the nucleus where it is phosphorylated by nuclear PKC delta, exported to the cytosol, dephosphorylated, and degraded. Intracellular FGF acidic inhibits p53 activity and proapoptotic signaling.

Synonym: Fibroblast Growth Factor 1, FGF-1, Acidic Fibroblast Growth Factor, aFGF, Endothelial Cell Growth Factor, ECGFHeparin-Binding Growth Factor 1, HBGF-1, FGF1, FGFA

Molekulargewicht: 15.9 kDa

UniProt: P05230

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung