EPH Receptor B2 Protein (EPHB2) (AA 570-987) (His-GST)

Produktnummer ABIN7317086

Recombinant EPH Receptor B2-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für EPH Receptor B2 Protein (EPHB2) (AA 570-987) (His-GST) (ABIN7317086)

Target: EPH Receptor B2 (EPHB2)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 570-987

Aufreinigungstag / Konjugat: Dieses EPH Receptor B2 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human EphB2 Protein (aa 570-987, His &GST Tag)

Sequenz: Gly570-Val987

Produktmerkmale: A DNA sequence encoding the human EPHB2 (P29323-3) (Gly570-Val987) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 120 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 3 mM DTT, 10 % glycerol

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: EPH Receptor B2 (EPHB2)

Andere Bezeichnung: EphB2

Hintergrund: CAPB,DRT,EK5,EPHB2,EPHT3,ERK,Ephrin type-B receptor 2,Hek5,PCBC,Tyro5,Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Molekulargewicht:

Calculated MW: 75.2 kDa

Observed MW: 65 kDa

Gen-ID: 2048

UniProt: P29323

Pathways: RTK Signalweg, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteine