EPH Receptor B1 Protein (EPHB1) (His-GST)

Produktnummer ABIN7320010

Dieses Recombinant EPH Receptor B1-Protein wird in Baculovirus infected Insect Cells produziert.

Kurzübersicht für EPH Receptor B1 Protein (EPHB1) (His-GST) (ABIN7320010)

Target: EPH Receptor B1 (EPHB1)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Reinheit: > 80 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses EPH Receptor B1 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Mouse EphB1/EPHT2 Protein (His & GST Tag)

Sequenz: Met 591-Ala 984

Produktmerkmale: A DNA sequence encoding the cytoplasmic domain (Met 591-Ala 984) of mouse EPHB1 (Q8CBF3-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 234 nmol/min/mg using Poly(Glu, Tyr) 4:1 as substrate.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: EPH Receptor B1 (EPHB1)

Andere Bezeichnung: EphB1/EPHT2

Hintergrund: 9330129L11,AW488255,C130099E04Rik,Cek6,ENSMUSG00000074119,Elk,Elkh,Hek6,Net,Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Molekulargewicht:

Calculated MW: 72.4 kDa

Observed MW: 60 kDa

UniProt: Q8CBF3

Pathways: RTK Signalweg