EPH Receptor A4 Protein (EPHA4) (AA 570-986) (His-GST)

Produktnummer ABIN7317076

Dieses Recombinant EPH Receptor A4-Protein wird in Baculovirus infected Insect Cells produziert.

Kurzübersicht für EPH Receptor A4 Protein (EPHA4) (AA 570-986) (His-GST) (ABIN7317076)

Target: EPH Receptor A4 (EPHA4)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: ELISA

Reinheit: > 99 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 570-986

Aufreinigungstag / Konjugat: Dieses EPH Receptor A4 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human EphA4 Protein (aa 570-986, His &GST Tag)

Sequenz: Ser 570-Val 986

Produktmerkmale: A DNA sequence encoding the human EPHA4 (P54764) (Ser570-Val986) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. The specific activity was determined to be 17 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Immobilized human EPHA4 (aa 570-986)at 10 μg/ml (100 μl/well) can bind biotinylated human EphrinA5-His with a linear range of 0.625-5.0 μg/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10 % glycerol, 3 mM DTT

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: EPH Receptor A4 (EPHA4)

Andere Bezeichnung: EphA4

Hintergrund: EK8;EPH-like kinase 8;EPHA4;Ephrin type-A receptor 4;HEK8;SEK;TYRO1;Tyrosine-protein kinase TYRO1;Tyrosine-protein kinase receptor SEK;hEK8,EPH receptor A4 (ephrin type-A receptor 4); also known as EphA4; belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1; EPHA2; EPHA3; EPHA4; EPHA5; EPHA6; EPHA7; EPHA8; EPHA9; EPHA10; EPHB1; EPHB2; EPHB3; EPHB4; EPHB5; EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus; and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling; which is triggered by ephrins7; probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development; forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands; which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.

Molekulargewicht:

Calculated MW: 75.0 kDa

Observed MW: 67 kDa

Gen-ID: 2043

UniProt: P54764

Pathways: RTK Signalweg