ERN1 Protein (AA 465-977) (His-GST)

Produktnummer ABIN7317060

Dieses Recombinant ERN1-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für ERN1 Protein (AA 465-977) (His-GST) (ABIN7317060)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 80 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 465-977

Aufreinigungstag / Konjugat: Dieses ERN1 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human ERN1/IRE1 Protein (aa 465-977, His & GST Tag)

Sequenz: Pro 465-Leu 977

Produktmerkmale: A DNA sequence encoding the human ERN1 isoform 1 (O75460-1) cytoplasmic domain (Pro 465-Leu 977) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 5 nmol/min/mg using MBP as substrate

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Andere Bezeichnung: ERN1/IRE1

Hintergrund: IRE1,IRE1P,IRE1a,hIRE1p,The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.

Molekulargewicht:

Calculated MW: 86.0 kDa

Observed MW: 90 kDa

Gen-ID: 2081

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response