Cyclin E1 Protein (CCNE1) (His-GST)

Produktnummer ABIN7317726

Recombinant Cyclin E1-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für Cyclin E1 Protein (CCNE1) (His-GST) (ABIN7317726)

Target: Cyclin E1 (CCNE1)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: ELISA

Reinheit: > 82 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses Cyclin E1 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human CCNE1/Cyclin-E1 Protein (His & GST Tag)

Sequenz: Met 1-Ala 410

Produktmerkmale: A DNA sequence encoding the full length of human CCNE1 isoform 1 (NP_001229.1) (Met 1-Ala 410) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized human HG3C-CCNE1 at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4, The EC₅₀ of biotinylated human GST-CDK4 is 45.0-106.0 ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, 1 mM GSH, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: Cyclin E1 (CCNE1)

Andere Bezeichnung: CCNE1/Cyclin-E1

Hintergrund: CCNE,Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.

Molekulargewicht:

Calculated MW: 75.0 kDa

Observed MW: 70 kDa

Gen-ID: 898

NCBI Accession: NP_001229

UniProt: P24864

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases