C5A Protein (AA 678-751)

Produktnummer ABIN7319677

Dieses Recombinant C5A-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für C5A Protein (AA 678-751) (ABIN7319677)

Target: C5A (C5a) (Complement Component 5a (C5a))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 678-751

Verwendungszweck: Recombinant Human Complement C5a Protein

Sequenz: Thr678-Arg751

Produktmerkmale: Recombinant Human C5a is produced by our E.coli expression system and the target gene encoding Thr678-Arg751 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: C5A (C5a) (Complement Component 5a (C5a))

Andere Bezeichnung: Complement C5a

Hintergrund: C5Da,C5a,C5a anaphylatoxin,CPAMD4,Complement C5,ECLZB,Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 α-chain. Human C5a has four α-helices plus three intrachain disulfide bonds that create a triple loop structure. Human C5a is 60 % and 54 % aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

Molekulargewicht:

Calculated MW: 8.4 kDa

Observed MW: 12 kDa

Gen-ID: 727

UniProt: P01031

Pathways: Komplementsystem, Carbohydrate Homeostasis