CDC37 Protein (AA 1-379) (His-GST)

Produktnummer ABIN7320133

Recombinant CDC37-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für CDC37 Protein (AA 1-379) (His-GST) (ABIN7320133)

Target: CDC37 (Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Reinheit: > 85 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-379

Aufreinigungstag / Konjugat: Dieses CDC37 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Mouse CDC37/CDC37A Protein (His & GST Tag)

Sequenz: Met1-Ala379

Produktmerkmale: A DNA sequence encoding the mouse CDC37 (Q61081) (Met1-Ala379) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM Nacl, 10 % glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: CDC37 (Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37))

Andere Bezeichnung: CDC37/CDC37A

Hintergrund: P50,p50Cdc37,CDC37 is a protein that is expressed in proliferative zones during embryonic development and in adult tissues, consistent with a positive role in proliferation and is required for cell division in budding yeast. CDC37 is though to play an important role in the establishment of signaling pathways controlling cell proliferation through targeting intrinsically unstable oncoprotein kinases such as Cdk-4, Raf-1, and src to the molecular chaperone Hsp90. Decreased Hsp90 expression can reduce the levels of microtubule-associated protein tau, whose overexpression may induce many diseases. CDC37 is considered as a co-chaperone that is classified to Hsp90's accessory proteins. It has been reported that suppression of Cdc37 destabilized tau, leading to its clearance, whereas cdc37 overexpression preserved tau.Cdc37 was found to co-localize with tau in neuronal cells and to physically interact with tau from human brain. Moreover, Cdc37 levels significantly increased with age.

Molekulargewicht:

Calculated MW: 72.4 kDa

Observed MW: 66 kDa

UniProt: Q61081