PD-L1 Protein (His tag)

Produktnummer ABIN7194779

Recombinant PD-L1-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für PD-L1 Protein (His tag) (ABIN7194779)

Target: PD-L1 (CD274 (PD-L1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Applikation: ELISA

Reinheit: > 98 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses PD-L1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse PD-L1/B7-H1/CD274 Protein (His Tag)

Sequenz: Met 1-Thr 238

Produktmerkmale: A DNA sequence encoding the mouse CD274 (NP_068693.1) extracellular domain (Met 1-Thr 238) was fused with a polyhistidine tag at the C-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized recombinant mouse PD1-L1 at 1 μg/ml (100 μl/well) can bind mouse PD1 with a linear range of 6. 25-400 ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: PD-L1 (CD274 (PD-L1))

Andere Bezeichnung: PD-L1/B7-H1/CD274

Hintergrund: A530045L16Rik Protein,B7h1 Protein,Mouse,Pdcd1l1 Protein,Pdcd1lg1 Protein,Pdl1 Protein,Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Molekulargewicht:

Calculated MW: 26.3 kDa

Observed MW: 40-45 kDa

NCBI Accession: NP_068693

UniProt: Q3U472

Pathways: Cancer Immune Checkpoints