PD-L1 Protein (DYKDDDDK Tag)

Produktnummer ABIN7318878

Recombinant PD-L1-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für PD-L1 Protein (DYKDDDDK Tag) (ABIN7318878)

Target: PD-L1 (CD274 (PD-L1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: ELISA, Bio-Layer Interferometry (BLI)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses PD-L1 Protein ist gelabelt mit DYKDDDDK Tag.

Verwendungszweck: Recombinant Human PD-L1/B7-H1/CD274 Protein (Flag Tag)

Sequenz: Phe19-Thr 239

Produktmerkmale: Recombinant Human Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a Flag tag at the C-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Loaded Human PD-1-Fc(PKSH033554) on Protein A Biosensor, can bind Human PD-L1-Flag(PKSH032870) with an affinity constant of 0.19 uM as determined in BLI assay.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: PD-L1 (CD274 (PD-L1))

Andere Bezeichnung: PD-L1/B7-H1/CD274

Hintergrund: B7 Homolog 1,B7-H1,B7H1,CD274,PD-L1,PDCD1 Ligand 1,PDCD1L1,PDCD1LG1,PDL1,Programmed Cell Death 1 Ligand 1,Programmed Death Ligand 1,CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Molekulargewicht:

Calculated MW: 26.3 kDa

Observed MW: 35-40 kDa

Gen-ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints