CPE Protein (Fc Tag)

Produktnummer ABIN7194611

Recombinant CPE-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für CPE Protein (Fc Tag) (ABIN7194611)

Target: CPE (Carboxypeptidase E (CPE))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 85 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses CPE Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Recombinant Human Carboxypeptidase E/CPE Protein (Fc Tag)

Sequenz: Met 1-Ser 453

Produktmerkmale: A DNA sequence encoding the human carboxypeptidase E (CPE) precursor (NP_001864.1) (Met 1-Ser 453) was expressed with the C-terminal fused Fc region of human IgG1.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: CPE (Carboxypeptidase E (CPE))

Andere Bezeichnung: Carboxypeptidase E/CPE

Hintergrund: Carboxypeptidase E(CPE for short),Carboxypeptidase H,Enkephalin convertase,Prohormone-processing carboxypeptidase,Carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.

Molekulargewicht:

Calculated MW: 74.6 kDa

Observed MW: 85-90 kDa

Gen-ID: 1363

NCBI Accession: NP_001864

UniProt: P16870

Pathways: Peptide Hormone Metabolism, Synaptic Membrane