BRD4 Protein

Produktnummer ABIN7319875

Dieses Recombinant BRD4-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für BRD4 Protein (ABIN7319875)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: >95 % as determined by reducing SDS-PAGE.

Produktdetails

Verwendungszweck: Recombinant Human BRD4 (N-10His-Flag)

Sequenz: Glu49-Glu460

Produktmerkmale: Recombinant Human Bromodomain-containing protein 4 is produced by our E.coli expression system and the target gene encoding Glu49-Glu460 is expressed with a 10His, Flag tag at the N-terminus.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 7.5.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Andere Bezeichnung: BRD4

Hintergrund:

Background: Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.

Synonym: HUNK1, HUNK1bromodomain-containing protein 4, HUNKI, MCAP

Molekulargewicht: 49 kDa

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interaktom