BID Protein (His-GST)

Produktnummer ABIN7320305

Recombinant BID-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für BID Protein (His-GST) (ABIN7320305)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: ELISA

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses BID Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Mouse BID Protein (His & GST Tag)

Sequenz: Met 1-Asp 195

Produktmerkmale: A DNA sequence encoding the mature form of mouse BID (EDK99650.1) (Met 1-Asp 195) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Biological Activity Comment: 1. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated human BCL2L1, The EC₅₀ of biotinylated human BCL2L1 is 7.01 ng/mL. 2. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC₅₀ of biotinylated mouse BCL2L1 is 7.1 ng/mL.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Andere Bezeichnung: BID

Hintergrund: 2700049M22Rik,AI875481,AU022477,The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.

Molekulargewicht:

Calculated MW: 50.0 kDa

Observed MW: 47 kDa

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity