AGO2 Protein (His tag)

Produktnummer ABIN7320195

Dieses Recombinant AGO2-Protein wird in Baculovirus infected Insect Cells produziert.

Kurzübersicht für AGO2 Protein (His tag) (ABIN7320195)

Target: AGO2 (Argonaute 2 (AGO2))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Reinheit: > 80 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses AGO2 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse AGO2/Argonaute 2/EIF2C2 Protein (His Tag)

Sequenz: Met 1-Ala 860

Produktmerkmale: A DNA sequence encoding the mouse AGO2 (Q8CJG0) (Met 1-Ala 860) was fused with a polyhistidine tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % Glycerol pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: AGO2 (Argonaute 2 (AGO2))

Andere Bezeichnung: AGO2/Argonaute 2/EIF2C2

Hintergrund: 1110029L17Rik,2310051F07Rik,AI225898,AL022874,AW546247,ENSMUSG00000072493,Eif2c2,Gerp95,Gm10365,mKIAA4215,Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 is a regulator of dFMR1 expression and has an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.

Molekulargewicht:

Calculated MW: 99.0 kDa

Observed MW: 105 kDa

UniProt: Q8CJG0

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Ribonucleoprotein Complex Subunit Organization