AGO2 Protein (DYKDDDDK Tag)

Produktnummer ABIN2669729

Dieses Recombinant AGO2-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für AGO2 Protein (DYKDDDDK Tag) (ABIN2669729)

Target: AGO2 (Argonaute 2 (AGO2))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Produktdetails

Aufreinigungstag / Konjugat: Dieses AGO2 Protein ist gelabelt mit DYKDDDDK Tag.

Verwendungszweck: Recombinant AGO2 protein

Produktmerkmale: Recombinant AGO2 (accession number NP_036286.2) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 104 kDa. The recombinant protein is >90 % pure by SDS-PAGE.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant AGO2 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: AGO2 (Argonaute 2 (AGO2))

Andere Bezeichnung: AGO2

Hintergrund:

Protein Background: AGO2 (Argonaute RISC Catalytic Component 2, also known as Q10, EIF2C2) is a member of the Argonaute family of proteins which play a role in RNA interference. This process is important both in gene regulation by microRNAs and in defense against viral infections. The RNA-induced silencing complex, or RISC, is a multiprotein complex that includes argonaute proteins and one strand of a small interfering RNA (siRNA) or microRNA (miRNA). RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleave the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'UTR). In addition, AGO2 can also up-regulate the posttranscriptional upregulation of specific mRNAs under certain growth conditions.

Short Description: Recombinant AGO2 (accession number NP_036286.2) was expressed in Sf9 cells and contains an N-terminal Flag tag with a molecular weight of 105 kDa. The recombinant protein is > 85 % pure by SDS-PAGE.

Aliases: Q10, EIF2C2

Molekulargewicht: 104 kDa

NCBI Accession: NP_036286

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Ribonucleoprotein Complex Subunit Organization