ANGPTL3 Protein (His tag)

Produktnummer ABIN7320853

Recombinant ANGPTL3-Protein exprimiert in Human Cells.

Kurzübersicht für ANGPTL3 Protein (His tag) (ABIN7320853)

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Human Cells

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses ANGPTL3 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse ANGPTL3 Protein (His Tag)

Sequenz: Ser17-Thr206

Produktmerkmale: Recombinant Mouse ANGPTL3 is produced by our Mammalian expression system and the target gene encoding Ser17-Thr206 is expressed with a 6His tag at the C-terminus.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Andere Bezeichnung: ANGPTL3

Hintergrund:

Background: Angiopoietin-likeProtein 3 (ANGPTL3) is a secreted glycoprotein that is structurally related to the angiopoietins. Mature mouse ANGPTL3 contains an N-terminalcoiled coil domain and a C-terminalfibrinogen-likedomain. Within the Nterminalfragment, mouse ANGPTL3 shares 83 % and 92 % aa sequence identity with human and rat ANGPTL3, respectively. ANGPTL3 is expressed in the liver from early in development through adulthood. ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymesresponsible for hydrolyzing circulating triglycerides and HDL phospholipids. This activity requires a putative heparin-bindingmotif which is N-terminalto thecoiled coil domain. Proteolytic removal of the fibrinogen-likedomain from the N-terminalfragment serves to activate ANGPTL3 and increase its ability toinhibit LPL in vitro and function in vivo. ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion oruptake. ANGPTL3 expression in vivo is up-regulatedby LXR agonists anddown-regulatedby insulin, leptin, and agonists of TRβ or PPARβ. ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells.

Synonym: Angiopoietin-related Protein 3, Angiopoietin-like protein 3, Angptl3

Molekulargewicht: 22.7 kDa

UniProt: Q9R182