Interferon gamma Protein (IFNG) (AA 24-166)

Produktnummer ABIN7123486

Recombinant Interferon gamma-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Interferon gamma Protein (IFNG) (AA 24-166) (ABIN7123486)

Target: Interferon gamma (IFNG)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), ELISA

Reinheit: Greater than 95 % as determined by SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 24-166

Verwendungszweck: Recombinant Human IFNG

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 0.01 EU/μg as determined by LAL test.

Biological Activity Comment: Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-0.7 ng/mL.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 5 % Sucrose, 4 % Mannitol, 0.02 % Tween 80, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Haltbarkeit: 12 months

Antigendetails

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFNG

Hintergrund: Interferon gamma, IFN-gamma, Immune interferon

Molekulargewicht: 16 kDa

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie