c-MET Protein (Fc Tag)

Produktnummer ABIN7121541

Dieses Recombinant c-MET-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für c-MET Protein (Fc Tag) (ABIN7121541)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: >95 % as determined by SDS-PAGE.

Güteklasse: MALS verified

Produktdetails

Aufreinigungstag / Konjugat: Dieses c-MET Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Mouse HGF R / c-MET Protein, Fc Tag (MALS verified)

Sequenz: Glu 25 - Ala 931

Produktmerkmale: Mouse HGF R, Fc Tag (MET-M5254) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Ala 931 (Accession # P16056-1).

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: Mouse HGF R, Fc Tag is fused with Fc fragment of human IgG1 at the C-terminus. The mature form of HGF R is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.1 kDa (α chain) and 95.1 kDa (β chain Fc chimera). The protein migrates as 40-45 kDa (α chain) and kDa (β chain Fc chimera) under reducing (R) condition due to glycosylation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Andere Bezeichnung: HGF R / c-MET

Hintergrund: Synonyms: MET,AUTS9,HGFR,RCCP2,c-Met,
Description: Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.

Molekulargewicht: 127.2 kDa

Pathways: RTK Signalweg, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor