SARS-CoV-2 Spike Protein (His-Avi Tag)

Produktnummer ABIN6953018

Dieses Recombinant SARS-CoV-2 Spike-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für SARS-CoV-2 Spike Protein (His-Avi Tag) (ABIN6953018)

Target: SARS-CoV-2 Spike

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Reinheit: >95 % as determined by SDS-PAGE and HPLC

Produktdetails

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike Protein ist gelabelt mit His-Avi Tag.

Produktmerkmale: DNA sequence encoding the COVID-19 Spike S protein extracellular domain,[16-1188] R683A (accession # QHD43416.1) in 3 replicates including a C-terminal His AVI tag was expressed in HEK Cells.

Endotoxin-Niveau: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: The activity was tested by binding Human ACE2 in functional ELISA assay

Anwendungsinformationen

Kommentare:

Recombinant 2019-nCoV Spike S trimer protein is a protein consisting of the S1+S2 subunits amino acid residues. The protein has a theoretical MW of 140kDa,however due to glycosylation migrates as an approximately 200 kDa protein on reduced SDS-PAGE.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: Recombinant nCoV-2019 Spike S trimer protein was lyophilized from 0.2 μm filtered PBS pH 7.4.

Lagerung: -20 °C

Informationen zur Lagerung: The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.

Antigendetails

Target: SARS-CoV-2 Spike

Substanzklasse: Viral Protein

Molekulargewicht: 140 kDa

Gen-ID: 43740568

UniProt: P0DTC2