SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (His tag,AVI tag,Biotin)

Produktnummer ABIN6952658

Produktdetails

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Protein-Typ: Recombinant

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Nucleocapsid Protein ist gelabelt mit His tag,AVI tag,Biotin.

Applikation: ELISA, SDS-PAGE (SDS)

Verwendungszweck: Biotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His,Avitag™

Sequenz: AA 1-419

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag is expressed from E.coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1). Predicted N-terminus: Met
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

Reinheit: >90 % as determined by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, Arginine, pH 7.5

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

Referenzen

Dogan, Kozhaya, Placek, Gunter, Yigit, Hardy, Plassmeyer, Coatney, Lillard, Bukhari, Kleinberg, Hayes, Arditi, Klapper, Merin, Liang, Gupta, Alpan, Unutmaz: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." in: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).

Antigendetails

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Andere Bezeichnung: SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N Produkte)

Substanzklasse: Viral Protein

Hintergrund: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Molekulargewicht: 51.1 kDa