ACE2 Protein (His tag,AVI tag,Biotin)

Produktnummer ABIN6952431

Produktdetails

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses ACE2 Protein ist gelabelt mit His tag,AVI tag,Biotin.

Applikation: SDS-PAGE (SDS), Functional Studies (Func)

Marke: MABSol®,PrecisionAvi

Sequenz: AA 18-740

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 87.2 kDa. The protein migrates as 95-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Reinheit: >90 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 50 mM Tris, 150 mM NaCl, pH 7.5

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen

Bell, Powell, Rodriguez, Cochran, Kim: "Neutralizing antibodies targeting the SARS-CoV-2 receptor binding domain isolated from a naïve human antibody library." in: Protein science : a publication of the Protein Society, Vol. 30, Issue 4, pp. 716-727, (2021) (PubMed).

Antigendetails

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Andere Bezeichnung: ACE2 (ACE2 Produkte)

Hintergrund: Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

Molekulargewicht: 87.2 kDa

NCBI Accession: NP_068576

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour