SARS-CoV-2 Spike S1 Protein (His tag)

Produktnummer ABIN6952427

Produktdetails

Target: SARS-CoV-2 Spike S1

Protein-Typ: Recombinant

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike S1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Functional Studies (Func)

Sequenz: AA 16-685

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76.9 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
AA 16-685

Reinheit: >90 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Biological Activity Comment: Immobilized 2019-nCoV S1 protein, His Tag at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 2-20 ng/mL (QC tested).

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen

Fan, Parr, Kang, Gupta: "Point-of-care (POC) SARS-CoV-2 antigen detection using functionalized aerosol jet-printed organic electrochemical transistors (OECTs)." in: Nanoscale, Vol. 15, Issue 11, pp. 5476-5485, (2023) (PubMed).

Lucas, Klein, Sundaram, Liu, Wong, Silva, Mao, Oh, Mohanty, Huang, Tokuyama, Lu, Venkataraman, Park, Israelow, Vogels, Muenker, Chang, Casanovas-Massana, Moore, Zell, Fournier, Wyllie, Campbell, Lee et al.: "Delayed production of neutralizing antibodies correlates with fatal COVID-19. ..." in: Nature medicine, (2021) (PubMed).

Song, Bartley, Chow, Ngo, Jiang, Zamecnik, Dandekar, Loudermilk, Dai, Liu, Sunshine, Liu, Wu, Hawes, Alvarenga, Huynh, McAlpine, Rahman, Geng, Chiarella, Goldman-Israelow, Vogels, Grubaugh et al.: "Divergent and self-reactive immune responses in the CNS of COVID-19 patients with neurological symptoms. ..." in: Cell reports. Medicine, pp. 100288, (2021) (PubMed).

Maritz, Woudberg, Bennett, Soares, Lapierre, Devine, Kimberg, Bouic: "Validation of high-throughput, semiquantitative solid phase SARS coronavirus-2 serology assays in serum and dried blood spot matrices." in: Bioanalysis, (2021) (PubMed).

Mazzini, Martinuzzi, Hyseni, Benincasa, Molesti, Casa, Lapini, Piu, Trombetta, Marchi, Razzano, Manenti, Montomoli: "Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples." in: Journal of immunological methods, Vol. 489, pp. 112937, (2021) (PubMed).

Haslwanter, Dieterle, Wec, OBrien, Sakharkar, Florez, Tong, Rappazzo, Lasso, Vergnolle, Wirchnianski, Bortz, Laudermilch, Fels, Mengotto, Malonis, Georgiev, Quiroz, Wrapp, Wang, Dye, Barnhill, Dye et al.: "A Combination of Receptor-Binding Domain and N-Terminal Domain Neutralizing Antibodies Limits the Generation of SARS-CoV-2 Spike Neutralization-Escape Mutants. ..." in: mBio, Vol. 12, Issue 5, pp. e0247321, (2021) (PubMed).

Pierce, Preston-Hurlburt, Dai, Aschner, Cheshenko, Galen, Garforth, Herrera, Jangra, Morano, Orner, Sy, Chandran, Dziura, Almo, Ring, Keller, Herold, Herold: "Immune responses to SARS-CoV-2 infection in hospitalized pediatric and adult patients." in: Science translational medicine, Vol. 12, Issue 564, (2020) (PubMed).

Luetkens, Metcalf, Planelles, Zheng, Larragoite, Spivak, Spivak, Steinbach, Blaylock, Avila, Hankey, Martins, Slev, Mannuel, Sajadi, Rapoport, Atanackovic: "Successful transfer of anti-SARS-CoV-2 immunity using convalescent plasma in an MM patient with hypogammaglobulinemia and COVID-19." in: Blood advances, Vol. 4, Issue 19, pp. 4864-4868, (2020) (PubMed).

Wei, Wan, Yan, Wang, Zhang, Yang, Zhang, Fan, Li, Deng, Sun, Gong, Yang, Wang, Wang, Li, Yang, Li, Zhang, Wang, Du, Zong, Yin, Zhang, Wang, Peng, Lin, Feng, Qin, Chen, Gao, Zhang, Cao, Zhong: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." in: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, (2020) (PubMed).

Antigendetails

Target: SARS-CoV-2 Spike S1

Abstract: SARS-CoV-2 Spike S1 Produkte

Substanzklasse: Viral Protein

Hintergrund: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molekulargewicht: 76.9 kDa

Gen-ID: 43740568

UniProt: P0DTC2