ERBB3 Protein (AA 20-643) (His tag,AVI tag,Biotin)

Produktnummer ABIN6933652

Dieses Recombinant ERBB3-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für ERBB3 Protein (AA 20-643) (His tag,AVI tag,Biotin) (ABIN6933652)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: >95 % as determined by reduced SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 20-643

Aufreinigungstag / Konjugat: Dieses ERBB3 Protein ist gelabelt mit His tag,AVI tag,Biotin.

Sequenz: AA 20-643

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Antigendetails

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Andere Bezeichnung: ErbB3

Hintergrund: ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Molekulargewicht: 72.4 kDa

NCBI Accession: NP_001973

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung