CCL4 Protein

Produktnummer ABIN6700901

Hersteller Produkt- Nr.: 010-001-b85

Dieses Recombinant CCL4-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für CCL4 Protein (ABIN6700901)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 95,00%

Produktdetails

Hersteller Produkt- Nr.: 010-001-b85

Hersteller: Rockland

Verwendungszweck: MIP-1ß Mouse Recombinant Protein

Aufreinigung: Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non- reducing SDS-PAGE.

Endotoxin-Niveau: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Mouse Macrophage Inflammatory Protein-1 beta is fully biologically active when compared to standard. The activity is calculated by the ability to chemoattract blood monocytes at 20-100 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: MIP-1ß protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-MIP-1ß in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Andere Bezeichnung: Ccl4

Hintergrund:

Synonyms: Mouse Macrophage Inflammatory Protein-1-beta (CCL4), C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, Immune activation protein 2, ACT-2, ACT2, Protein H400, SIS-gamma, Mip1b, Scya4

Background: MIP-1ß is a secreted monokine with inflammatory and chemokinetic properties and can exist as a homodimer. It belongs to the intercrine beta (chemokine CC) family. Recombinant Mouse Macrophage Inflammatory Protein-1 beta produced in E. coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7,808 Daltons.

UniProt: P14097