YWHAE Protein (GST tag)

Produktnummer ABIN6700778

Hersteller Produkt- Nr.: 009-001-r25s

Dieses Recombinant YWHAE-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für YWHAE Protein (GST tag) (ABIN6700778)

Target: YWHAE (14-3-3 epsilon (YWHAE))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Reinheit: >90%

Produktdetails

Aufreinigungstag / Konjugat: Dieses YWHAE Protein ist gelabelt mit GST tag.

Hersteller Produkt- Nr.: 009-001-r25s

Hersteller: Rockland

Verwendungszweck: 14-3-3 epsilon recombinant protein-GST fusion protein

Aufreinigung: Recombinant full-length human 14-3-3ε was expressed in E.coli cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Anwendungsinformationen

Applikationshinweise:

Application Note: 14-3-3ε Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3ε Protein is suitable for use in Western Blot. Expect a band approximately ~55 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: 14-3-3ε Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: YWHAE (14-3-3 epsilon (YWHAE))

Andere Bezeichnung: YWHAE

Hintergrund:

Synonyms: 14-3-3 epsilon, YWHAE, MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465

Background: 14-3-3ε also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein is a member of the 14-3-3 family. 14-3-3ε has been reported to be involved in heat shock transcription factor 1 (HSF1) and extracellular signal regulated protein kinase (ERK) pathways (1). 14-3-3ε interaction with the deubiquitinating enzyme UBPY, also known as USP8, regulates cargo sorting and membrane traffic at early endosomes. Association of 14-3-3ε, HSF1 with ERK during heat shock may modulate the amplitude of the response and control the termination of HSP expression on resumption of growth conditions (2). 14-3-3ε protein is ideal for investigators involved in Cell Stress & Chaperone Proteins, Cell Signaling, Cancer research, Cell Cycle, Cellular Stress, Neurobiology, and WNT Signaling research.

NCBI Accession: NM_006761

Pathways: Neurotrophin Signalübertragung, Myometrial Relaxation and Contraction, M Phase