BAFF Protein

Produktnummer ABIN6700762

Hersteller Produkt- Nr.: 009-001-u65-0020

Dieses Recombinant BAFF-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für BAFF Protein (ABIN6700762)

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 90,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-u65-0020

Hersteller: Rockland

Verwendungszweck: Human BAFF

Aufreinigung: BAFF purity was determined to be greater than 90% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by a RPMI-8226 cell survival assay in the presence of 0.2 μM DEX and is typically less than 30 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Human BAFF Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-BAFF in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Andere Bezeichnung: TNFSF13B

Hintergrund:

Synonyms: THANK, B lymphocyte stimulator (BLyS), B-cell activating factor, TNF- and APOL-related leukocyte expressed ligand 1 (TALL-1), CD_antigen=CD257

Background: Human B cell Activating Factor (BAFF), is a Type II member of the TNF superfamily. Although it is typically a transmembrane protein expressed on T cells, macrophages and dendritic cells, it can also be cleaved in the extracellular region to produce a soluble form detectable in the serum. BAFF is thought to be important for the survival and maturation of peripheral B cells. BAFF initiates signaling through three receptors with human BAFF having activity on mouse splenocytes. Recombinant human BAFF is a non-glycosylated protein containing a N terminal His tag, a thrombin cut site and 153 amino acids of the TNF-like domain. This protein has a total molecular weight of 18.5 kDa.

UniProt: Q9Y275

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response