Stathmin 1 Protein (STMN1) (His tag)

Produktnummer ABIN6700567

Hersteller Produkt- Nr.: 009-001-t58s

Dieses Recombinant Stathmin 1-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Stathmin 1 Protein (STMN1) (His tag) (ABIN6700567)

Target: Stathmin 1 (STMN1)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Reinheit: >90%

Produktdetails

Aufreinigungstag / Konjugat: Dieses Stathmin 1 Protein ist gelabelt mit His tag.

Hersteller Produkt- Nr.: 009-001-t58s

Hersteller: Rockland

Verwendungszweck: Stathmin recombinant protein-HIS Epitope

Aufreinigung: Recombinant full-length human Stathmin protein was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >90% by densitometry.

Anwendungsinformationen

Applikationshinweise:

Application Note: Stathmin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. Stathmin Protein is suitable for use in Western Blot. Expect a band approximately ~19 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: Stathmin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Stathmin 1 (STMN1)

Andere Bezeichnung: STMN1

Hintergrund:

Synonyms: STMN1, C1orf215, FLJ32206, Lag, LAP18, MGC138869, MGC138870, OP18, PP17, PP19, PR22, SMN, Stathmin

Background: Stathmin is a ubiquitous, highly conserved protein present in the cytoplasm in a variety of unphosphorylated and phosphorylated forms. Stathmin functions as an intracellular relay integrating regulatory signals of the cellular environment (1). Stathmin is involved in the regulation of the microtubule filament system. Stathmin binds tubulin in a ternary complex, with 2 tubulins for every stathmin and this complex interferes with microtubule dynamics in vitro and in vivo. Overexpression of several stathmin mutants suggest that stathmin induces depolymerization of interphase and mitotic microtubules in its unphosphorylated state, but is inactivated by phosphorylation in mitosis (2). Stathmin Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Cancer, Invasion/Metastasis, and Neurobiology research.

Pathways: MAPK Signalweg, Microtubule Dynamics