IL-4 Protein

Produktnummer ABIN6700136

Hersteller Produkt- Nr.: 009-f01-b93-0005

Dieses Recombinant IL-4-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für IL-4 Protein (ABIN6700136)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 98,00%

Güteklasse: Animal-Free

Produktdetails

Hersteller Produkt- Nr.: 009-f01-b93-0005

Hersteller: Rockland

Verwendungszweck: Human Interleukin-4 Recombinant Protein (Animal Free)

Aufreinigung: Interleukin-4 is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 98% as determined by HPLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-PAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by a proliferation assay using MC-9 cells and is typically less than 0.2 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Interleukin-4 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-4 in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: IL4

Hintergrund:

Synonyms: B cell Stimulating Factor (BSF-1), Binetrakin, BCDF, BCGF, Lymphocyte stimulatory factor 1, Pitrakinra

Background: Interleukin-4 (IL-4) is an immuno-modulatory cytokine, produced primarily by a subgroup of helper T cells, known as Th2. IL-4 is known to induce naive helper T cells to differentiate to Th2 cells, which subsequently produce more IL-4. Th2 cells regulate a wide variety of other immune cells including, B cells, where it has the ability to promote IgG to IgE isotype switching. Recombinant human IL-4 is a non-glycosylated protein, containing 130 amino acids, with a molecular weight of 15 kDa.

UniProt: P05112

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation