GM-CSF Protein

Produktnummer ABIN6699896

Hersteller Produkt- Nr.: 010-001-314

Dieses Recombinant GM-CSF-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für GM-CSF Protein (ABIN6699896)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 95,00%

Produktdetails

Hersteller Produkt- Nr.: 010-001-314

Hersteller: Rockland

Verwendungszweck: GM-CSF Mouse Recombinant Protein

Aufreinigung: Purity greater than 95% as determined by analysis by RP-HPLC and reducing and non- reducing SDS-PAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: GM-CSF activity is determined by the dose-dependent proliferation of mouse FDCP-1 cell line and is typically less than 10 pg/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: GM-CSF protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-GM-CSF in immunological assays. Lyophilized in 10 mM acetic Acid.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Konzentration: 0.2 mg/mL

Buffer: Lyophilized in 10 mM acetic Acid.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Andere Bezeichnung: Csf2

Hintergrund:

Synonyms: Granulocyte Macrophage Colony Stimulating Factor, Granulocyte Macrophage-CSF, Granulocyte-macrophage colony-stimulating factor, GM-CSF cytokine, Colony-stimulating factor, CSF, CSF2, Sargramostim, Molgramostin

Background: M-GM-CSF is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL2 -beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors. Recombinant mouse GM- CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14,285.35 Daltons.

UniProt: Q14AD9

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin