Follistatin Protein (FST)

Produktnummer ABIN6699880

Hersteller Produkt- Nr.: 009-001-u88-0100

Dieses Recombinant Follistatin-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Follistatin Protein (FST) (ABIN6699880)

Target: Follistatin (FST)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 95,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-u88-0100

Hersteller: Rockland

Verwendungszweck: Human Follistatin Recombinant Protein

Aufreinigung: Follistatin purity was determined to be greater than 95% as determined by analysis by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent neutralization of 7.5 ng/mL human Activin A. Complete neutralization is typically reached at less than 0.3 ug/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Follistatin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Follistatin in immunological assays. This product is provided as a lyophilized powder, in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer: This product is provided as a lyophilized powder, in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: Follistatin (FST)

Andere Bezeichnung: FST

Hintergrund:

Synonyms: FS, activin-binding protein, FSH-suppressing protein (FSP)

Background: Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-beta family members (activin, BMPs, myostatin, GDF-11 and TGF-beta 1). Mechanistically, follistatin works as an antagonist by complexing with TGF-beta family members to prevent them from interacting with their signaling receptors. Recombinant human Follistatin is a non-glycosylated protein, containing 289 amino acids, with a molecular weight of 31.7 kDa.

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion