FGF21 Protein

Produktnummer ABIN6699850

Hersteller Produkt- Nr.: 009-001-u77-0005

Dieses Recombinant FGF21-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für FGF21 Protein (ABIN6699850)

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 95,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-u77-0005

Hersteller: Rockland

Verwendungszweck: Human Fibroblast Growth Factor-21 Recombinant Protein

Aufreinigung: Fibroblast Growth Factor-21 purity was determined to be greater than 95% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of NIH 3T3 cells in the presence of 5 μg/mL FGF binding protein and is typically 0.12-0.6 μg/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Fibroblast Growth Factor-21 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor-21 in immunological assays. Lyophilized in 0.1M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer: Lyophilized in 0.1M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Andere Bezeichnung: FGF21

Hintergrund:

Synonyms: FGFL

Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. FGF-21 is most closely related to FGF-19 and shows low binding to heparin and is highly diffusible. Recombinant human FGF-21 is a non-glycosylated protein, containing 182 amino acids, with a molecular weight of 19.5 kDa.

UniProt: Q9NSA1

Pathways: RTK Signalweg